Regulation of Xanthine Oxidoreductase Protein Expression by Hydrogen Peroxide and Calcium

Regulation of xanthine oxidoreductase protein expression by hydrogen peroxide and calcium.

OBJECTIVE We have previously demonstrated that endothelial xanthine oxidase (XO) levels are dependent on the NADPH oxidase. We postulated that H2O2 may modulate the irreversible conversion of xanthine dehydrogenase (XDH) to XO and sought to examine mechanisms involved. METHODS AND RESULTS H2O2 (100 micromol/L) decreased bovine aortic endothelial cell (BAEC) XDH protein expression, and metabol...

Expression and Regulation of Human Xanthine Oxidoreductase

Yliopistopaino The truth is rarely pure and never simple.

Regulation of xanthine oxidoreductase by intracellular iron.

Xanthine oxidoreductase (XOR) may produce reactive oxygen species and play a role in ischemia-reperfusion injury. Because tissue iron levels increase after ischemia, and because XOR contains functionally critical iron-sulfur clusters, we studied the effects of intracellular iron on XOR expression. Ferric ammonium citrate and FeSO(4) elevated intracellular iron levels and increased XOR activity ...

Regulation of the Human Xanthine Oxidoreductase Gene

Regulation of hydrogen peroxide production by brain mitochondria by calcium and Bax.

Abnormal accumulation of Ca2+ and exposure to pro-apoptotic proteins, such as Bax, is believed to stimulate mitochondrial generation of reactive oxygen species (ROS) and contribute to neural cell death during acute ischemic and traumatic brain injury, and in neurodegenerative diseases, e.g. Parkinson's disease. However, the mechanism by which Ca2+ or apoptotic proteins stimulate mitochondrial R...